Researchers make a new discovery in age-related macular degeneration

Summary: Treatment with Humanin G reduced protein levels of markers of inflammation that become elevated in age-related macular degeneration.

Source: Impact reviews

Inflammatory processes drive the progression of age-related macular degeneration (AMD), one of the leading causes of vision loss in the United States.

In this new Aging study, researchers from the University of California, Irvine and the University of Southern California compared protein levels of inflammation markers in normal and AMD transmitochondrial cybrid cells of the retinal pigment epithelium (RPE) and studied the effects of treatment with exogenous Humanin G.

Humanin G (HNG) is a mitochondria-derived peptide that is cytoprotective in AMD and may protect against mitochondrial and cellular stress induced by damaged mitochondria in AMD.

“The purpose of this study was to test our hypothesis that the levels of marker proteins associated with inflammation are increased in AMD and treatment with HNG leads to a reduction in their protein levels.”

Humanin G protein levels were measured in the plasma of AMD patients and normal subjects using an ELISA test. Humanin G was added to AMD and normal cybrids (controls) derived from clinically characterized AMD patients and normal subjects (controls).

Cell lysates were extracted from untreated and HNG-treated normal AMD and cybrids, and the Luminex XMAP multiplex assay was used to measure inflammatory protein levels.

The researchers found that there were differential levels of inflammatory proteins between normal and AMD plasma samples. Compared to control plasma samples, AMD plasma showed higher protein levels of inflammation markers.

Inflammatory processes drive the progression of age-related macular degeneration (AMD), one of the leading causes of vision loss in the United States. Image is in public domain

However, plasma levels of endogenous humanin protein were 36.58% lower in patients with AMD than in age-matched normal subjects. After treatment with Humanin G, researchers observed a marked reduction in protein levels of markers of inflammation that were elevated in AMD RPE transmisochondrial cybrid cells.

“In conclusion, we present new findings that: a) show reduced levels of Humanin proteins in AMD plasma compared to normal plasma; b) suggest the role of inflammatory markers in the pathogenesis of AMD, and c) highlight the positive effects of Humanin G in reducing inflammation in AMD.

To the teams’ knowledge, this is the first study to report significantly reduced Humanin protein levels in AMD patients, supporting the central role of Humanin in maintaining tissue homeostasis and function. normal eye.

“Our discovery is novel and could contribute to the development of therapeutic tools to reduce inflammation in order to alleviate the pathology of AMD”, conclude the researchers.

About this Age-Related Macular Degeneration Research News

Author: Press office
Source: Impact reviews
Contact: Press office – Impact reviews
Image: Image is in public domain

Original research: Free access.
“Effect of Humanine G (HNG) on inflammation in age-related macular degeneration (AMD)” by Sonali Nashine et al. Aging

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Summary

Effect of Humanine G (HNG) on inflammation in age-related macular degeneration (AMD)

Inflammation plays a crucial role in the etiology and pathogenesis of AMD (age-related macular degeneration). Humanin G (HNG) is a mitochondrial-derived peptide (MDP) that is cytoprotective in AMD and may protect against mitochondrial and cellular stress induced by damaged mitochondria in AMD.

The aim of this study was to test our hypothesis that the levels of marker proteins associated with inflammation are increased in AMD and treatment with HNG leads to a reduction in their protein levels. Humanine protein levels were measured in the plasma of AMD patients and normal subjects using an ELISA test. Humanin G was added to AMD and normal cybrids (controls) that had identical nuclei from mitochondria-deficient ARPE-19 cells but differed in mitochondrial DNA (mtDNA) content derived from clinically-derived AMD patients. characterized and normal subjects (controls).

Cell lysates were extracted from untreated and HNG-treated normal AMD and cybrids, and the Luminex XMAP multiplex assay was used to measure inflammatory protein levels. AMD plasma showed reduced levels of Humanin protein, but higher protein levels of inflammation markers compared to control plasma samples.

In AMD RPE cybrid cells, humanin G reduced CD62E/E-selectin, CD62P/P-selectin, ICAM-1, TNF-α, MIP-1α, IFN-γ, IL-1β, IL-13 and IL-17A protein levels, suggesting that Humanin G can rescue from mtDNA-mediated inflammation in AMD cybrids.

In conclusion, we present new findings that: A) show reduced levels of Humanine protein in AMD plasma compared to normal plasma; B) suggest the role of inflammatory markers in the pathogenesis of AMD, and C) highlight the positive effects of Humanin G in reducing inflammation in AMD.

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